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To rule out the possibility that the GFP-NUDF protein was degraded in the double mutant, we performed a Western analysis with total protein extracts, and there was no significant reduction in the level of GFP-NUDF in this double mutant Figure 5B. Aspergillus nidulans apsA anucleate primary sterigmata encodes a coiled-coil protein required for nuclear positioning and completion of asexual development. Because of the opposite effects on microtubules that failed to reach the hyphal tip and those that had reached the hyphal tip, on average there was no dramatic change in the total frequency of catastrophe. Most microtubules in A. The Coils server http: Get the latest TV news from Entertainment Weekly Please Enter a Valid Email Address Please Enter a Valid ZIP Address.
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